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Leucocytes pp 65-86 | Cite as

Analyzing Cell Death Events in Cultured Leukocytes

  • Karin Christenson
  • Fredrik B. Thorén
  • Johan BylundEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 844)

Abstract

Cell death is of utmost importance in immunity, in part as a way to control the development and activity of leukocytes, but also as a strategy employed by leukocytes to rid the body of unwanted cells. Apoptosis is the classic type of programmed cell death involving an ordered sequence of cellular events, resulting in morphological changes that include cleavage/fragmentation of DNA, condensation of nuclei, cell shrinkage, and alterations of the plasma membrane. The apoptotic cell is a nonfunctional, but structurally intact, entity with preserved membrane integrity that is engulfed by surrounding cells (a process known as clearance) in an immunologically silent manner. In contrast, necrotic cells, i.e., nonfunctional cells that have lost membrane integrity, are freely permeable and leak intracellular constituents that may shift immunological homeostasis. Thus, membrane integrity of dead leukocytes is very important from an immunological point of view.

For the analysis of leukocyte cell death, a wide variety of assays are available to monitor different events along the cell death pathway; a combination of different methods is advantageous in order to gain a more complete understanding of this dynamic process. In this chapter, we describe several in vitro methods for evaluating leukocyte cell death, mainly focusing on apoptosis in human neutrophils and lymphocytes. Special emphasis is given to assessment of membrane integrity of the cultured cells. Furthermore, a protocol for monitoring clearance of apoptotic neutrophils by monocyte-derived macrophages is provided.

Key words

Cell death Apoptosis Necrosis Leukocytes Neutrophils Phagocytosis Inflammation 

Notes

Acknowledgments

This work was supported by the Swedish Research Council, the King Gustav V Memorial Foundation, Ingabritt and Arne Lundgren’s Research Foundation, Gunvor and Ivan Svensson’s Foundation, and the Swedish state under the LUA/ALF agreement. F.B. Thorén was supported by EMBO and the European commission (Marie Curie Intra-European Fellowship).

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Karin Christenson
    • 1
  • Fredrik B. Thorén
    • 2
  • Johan Bylund
    • 1
    Email author
  1. 1.Phagocyte Research Lab, Department of Rheumatology and Inflammation ResearchSahlgrenska Academy at University of GothenburgGothenburgSweden
  2. 2.Section of Hematology, Department of Internal MedicineSahlgrenska Academy at University of GothenburgGothenburgSweden

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