Leucocytes pp 15-30

Part of the Methods in Molecular Biology book series (MIMB, volume 844)

Detection and Quantification of Cytokines and Other Biomarkers

  • Evan L. Chiswick
  • Elizabeth Duffy
  • Brian Japp
  • Daniel Remick
Protocol

Abstract

Accurate measurement of cytokine concentrations is a powerful and essential approach to the study of inflammation. The enzyme-linked immunosorbent assay (ELISA) is a simple, low-cost analytical tool that provides both the specificity and sensitivity required for the study of cytokines in vitro or in vivo. This communication describes a systematic approach to develop an indirect sandwich ELISA to detect and quantify cytokines, or other biomarkers, with accuracy and precision. Also detailed is the use of sequential ELISA assays to analyze multiple cytokines from samples with limited volumes. Finally, the concept of a multiplex ELISA is discussed with considerations given to cost and additional time required for development.

Key words

Cytokines Sandwich ELISA Sequential ELISA Multiplex ELISA Antibodies 

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Evan L. Chiswick
    • 1
  • Elizabeth Duffy
    • 1
  • Brian Japp
    • 1
  • Daniel Remick
    • 1
  1. 1.Department of Pathology and Laboratory MedicineBoston University School of Medicine and Boston Medical CenterBostonUSA

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