Leucocytes pp 183-187

Part of the Methods in Molecular Biology book series (MIMB, volume 844)

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Isolation and Differentiation of Monocytes–Macrophages from Human Blood

Protocol

Abstract

The prevalence of fungal infections remains high, and it is associated with significant mortality and morbidity. Macrophages are heterogeneous population of effectors enriched in regions of Candida colonization. These cells sense Candida, and are critical in the resolution of these infections. Here, we describe how macrophages are generated in the presence of colony-stimulating factor-1 (CSF-1); an important cytokine required for the survival, proliferation and ex-vivo differentiation of monocytes to macrophages.

Key words

Macrophages CSF-1 Candida infections Monocyte differentiation MACs separation 

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  1. 1.Australian Institute for Bioengineering and NanotechnologyThe University of QueenslandSt LuciaAustralia

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