Cardiovascular Development pp 69-78

Part of the Methods in Molecular Biology book series (MIMB, volume 843)

Whole-Mount Confocal Microscopy for Vascular Branching Morphogenesis

  • Yoh-suke Mukouyama
  • Jennifer James
  • Joseph Nam
  • Yutaka Uchida
Protocol

Abstract

We introduce a whole-mount immunohistochemistry method for analyzing intricate vascular network formation in mouse embryonic tissues. Laser scanning confocal microscopy with multiple labeling allows for robust imaging of blood and lymphatic vessel branching morphogenesis with excellent resolution.

Key words

Confocal microscopy Whole-mount immunohistochemistry Mouse embryo Blood vessel Lymphatic vessel Fluorescence Antibody Patterning 

References

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Yoh-suke Mukouyama
    • 1
  • Jennifer James
    • 1
  • Joseph Nam
    • 1
  • Yutaka Uchida
    • 1
  1. 1.Laboratory of Stem Cell and Neuro-Vascular Biology, Genetics and Developmental Biology CenterNational Heart, Lung, and Blood Institute, National Institutes of HealthBethesdaUSA

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