Chicken Chorioallantoic Membrane Angiogenesis Model

  • Domenico RibattiEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 843)


The chick embryo chorioallantoic membrane (CAM) is an extraembryonic membrane which serves as a gas exchange surface and its function is supported by a dense capillary network. Because of its extensive vascularization and easy accessibility, the CAM has been broadly used to study the morphofunctional aspects of the angiogenesis process in vivo and to investigate the efficacy and mechanisms of action of proangiogenic and antiangiogenic natural and synthetic molecules. The CAM has long been a favored system for the study of tumor angiogenesis and metastasis, because at this stage the chick immunocompetence system is not fully developed and the conditions for rejection have not been established. The CAM may also be used to verify the ability to inhibit the growth of capillaries by implanting tumors onto the CAM and by comparing tumor growth and vascularization with or without the administration of an antiangiogenic molecule. Other studies using the tumor cells/CAM model have focused on the invasion of the chorionic epithelium and the blood vessels by tumor cells. The cells invade the epithelium and the mesenchymal connective tissue below, where they are found in the form of a dense bed of blood vessels, which is a target for intravasation.

Key words

Angiogenesis Antiangiogenesis Chorioallantoic membrane 



Supported in part by MIUR (PRIN 2007), Rome, and Fondazione Cassa di Risparmio di Puglia, Bari, Italy.


  1. 1.
    Ribatti, D. (2008) Chick embryo chorioallantoic membrane as a useful tool to study angiogenesis, Int Rev Cell Mol Biol 270, 181–224.PubMedCrossRefGoogle Scholar
  2. 2.
    Cimpean, A. M., Ribatti, D., and Raica, M. (2008) The chick embryo chorioallantoic membrane as a model to study tumor metastasis, Angiogenesis 11, 311–319.PubMedCrossRefGoogle Scholar
  3. 3.
    Deryugina, E. I., and Quigley, J. P. (2008) Chick embryo chorioallantoic membrane model systems to study and visualize human tumor cell metastasis, Histochem Cell Biol 130, 1119–1130.PubMedCrossRefGoogle Scholar
  4. 4.
    Ausprunk, D. H., Knighton, D. R., and Folkman, J. (1974) Differentiation of vascular endothelium in the chick chorioallantois: a structural and autoradiographic study, Dev Biol 38, 237–248.PubMedCrossRefGoogle Scholar
  5. 5.
    Ribatti, D. (2010) Different Morphological Techniques and Methods of Quantifying the Angiogenic Response Used in the Study of Vascularization in the Chorioallantoic Membrane, in The chick embryo chorioallantoic membrane in the study of angiogenesis and metastasis. (Ribatti, D., Ed) pp 71-74 Springer, New York.Google Scholar
  6. 6.
    Knighton, D. R., Fiegel, V. D., and Phillips, G. D. (1991) The assay of angiogenesis, Prog Clin Biol Res 365, 291–299.PubMedGoogle Scholar
  7. 7.
    Jakob, W., Jentzsch, K. D., Mauersberger, B., and Heder, G. (1978) The chick embryo choriallantoic membrane as a bioassay for angiogenesis factors: reactions induced by carrier materials, Exp Pathol (Jena) 15, 241–249.Google Scholar

Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  1. 1.Department of Basic Medical Sciences, Section of Human Anatomy and HistologyUniversity of Bari Medical School, PoliclinicoBariItaly

Personalised recommendations