Ancient DNA pp 57-63 | Cite as

Ancient DNA Extraction from Soils and Sediments

  • James HaileEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 840)


DNA contained in soils and sediments can provide novel insights into past environments and ecosystems. In this chapter, I describe an efficient and effective technique to extract total DNA from sediments in a manner that minimizes the coextraction of PCR-inhibitory compounds. I describe two different approaches: one that is suitable for large (up to 10 g wet weight) amounts of substrate, and a second that is more appropriate for small (up to 0.5 g) amounts of substrate. Finally, I discuss some of the obstacles that may be encountered in the process of extracting DNA from soils and sediments and suggest approaches to circumvent some common problems.

Key words

sediment soils ancient DNA metagenomics environmental sampling sedaDNA 



This work was supported by Murdoch University, Perth, Australia.


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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  1. 1.Ancient DNA Laboratory, School of Biological Sciences and BiotechnologyMurdoch UniversityPerthAustralia

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