Ancient DNA Extraction from Soils and Sediments
DNA contained in soils and sediments can provide novel insights into past environments and ecosystems. In this chapter, I describe an efficient and effective technique to extract total DNA from sediments in a manner that minimizes the coextraction of PCR-inhibitory compounds. I describe two different approaches: one that is suitable for large (up to 10 g wet weight) amounts of substrate, and a second that is more appropriate for small (up to 0.5 g) amounts of substrate. Finally, I discuss some of the obstacles that may be encountered in the process of extracting DNA from soils and sediments and suggest approaches to circumvent some common problems.
Key wordssediment soils ancient DNA metagenomics environmental sampling sedaDNA
This work was supported by Murdoch University, Perth, Australia.
- 9.Bulat S, Lubeck M, Alekhina I, Jensen F, Knudsen I, Lubeck P (2000) Identification of a universally primed-PCR-derived sequence-characterized amplified region marker for an antagonistic strain of Clonostachys rosea and development of a strain-specific PCR detection assay. Appl Environ Microbiol 66:4758–4763PubMedCrossRefGoogle Scholar
- 10.Voet D, Voet J (1995) Biochemistry. Wiley, New YorkGoogle Scholar