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Ancient DNA pp 43-49 | Cite as

DNA Extraction from Keratin and Chitin

  • Paula F. Campos
  • Thomas M. P. GilbertEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 840)

Abstract

DNA extracted from keratinous and chitinous materials can be a useful source of genetic information. To effectively liberate the DNA from these materials, buffers containing relatively high levels of DTT, proteinase K, and detergent are recommended, followed by purification using either silica-column or organic methods.

Key words

Chitinous tissue Keratinous tissue Silica DNA extraction Ancient DNA hair feathers nail cuticle exoskeleton 

Notes

Acknowledgments

MTPG was supported by the Danish National Science Foundation’s “Skou” grant program.

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  1. 1.Natural History Museum of DenmarkUniversity of CopenhagenCopenhagenDenmark
  2. 2.Natural History Museum of DenmarkUniversity of CopenhagenCopenhagenDenmark

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