In Silico Identification and Characterization of Effector Catalogs
Many characterized fungal effector proteins are small secreted proteins. Effectors are defined as those proteins that alter host cell structure and/or function by facilitating pathogen infection. The identification of effectors by molecular and cell biology techniques is a difficult task. However, with the availability of whole-genome sequences, these proteins can now be predicted in silico. Here, we describe in detail how to identify and characterize effectors from a defined fungal proteome using in silico techniques.
Key wordsSecretome Effector Pathogen Host Interaction PHI-base SignalP InterProScan GO Terms WoLF PSORT
This research was supported by a Vidi grant of the Research Council for Earth and Life Sciences (ALW) of the Netherlands Organization for Scientific Research (NWO), by the European Research Area–Network (ERA-NET) Plant Genomics and by the Centre for BioSystems Genomics (CBSG), which is part of the Netherlands Genomics Initiative and NWO.
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