Serglycin: The Master of the Mast Cell
Serglycin is a proteoglycan composed of a relatively small (∼17 kDa) core protein to which sulfated glycosaminoglycans of either heparin, heparan sulfate or chondroitin sulfate types are attached. Serglycin is expressed in many cell types, including in particular cells of hematopoietic origin. To study the function of serglycin, we have used a serglycin knockout mouse strain. A striking finding was that the mast cell population was severely affected by the absence of serglycin, as evidenced by distorted granule morphology and defective staining with cationic dyes. Moreover, the absence of serglycin caused a dramatic effect on the ability of mast cells to store a number of granule compounds, including several mast cell-specific proteases as well as biogenic amines. Hence, serglycin has a major function in maintaining mast cell secretory granule homeostasis.
Key wordsSerglycin Proteoglycans Mast cells Granules Secretion
Bone marrow-derived mast cell
Mouse mast cell protease
Stem cell factor
Mast cell carboxypeptidase A
Hypoxanthine guanine phosphoribosyl transferase
The authors of this chapter receive support from The Swedish Research Council, Formas, King Gustaf V’s 80-year Anniversary Fund, Torsten and Ragnar Söderberg Foundation, and The Swedish Cancer Foundation.
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