Proteoglycans pp 201-217 | Cite as

Serglycin: The Master of the Mast Cell

  • Elin Rönnberg
  • Gunnar PejlerEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 836)


Serglycin is a proteoglycan composed of a relatively small (∼17 kDa) core protein to which sulfated glycosaminoglycans of either heparin, heparan sulfate or chondroitin sulfate types are attached. Serglycin is expressed in many cell types, including in particular cells of hematopoietic origin. To study the function of serglycin, we have used a serglycin knockout mouse strain. A striking finding was that the mast cell population was severely affected by the absence of serglycin, as evidenced by distorted granule morphology and defective staining with cationic dyes. Moreover, the absence of serglycin caused a dramatic effect on the ability of mast cells to store a number of granule compounds, including several mast cell-specific proteases as well as biogenic amines. Hence, serglycin has a major function in maintaining mast cell secretory granule homeostasis.

Key words

Serglycin Proteoglycans Mast cells Granules Secretion 



Mast cell


Bone marrow-derived mast cell


Mouse mast cell protease


Chondroitin sulfate






Stem cell factor


Mast cell carboxypeptidase A


Hypoxanthine guanine phosphoribosyl transferase






Chondroitin 4-sulfotransferase




Chondroitin 6-sulfotransferase


Glucuronic acid


Iduronic acid


Wild type



The authors of this chapter receive support from The Swedish Research Council, Formas, King Gustaf V’s 80-year Anniversary Fund, Torsten and Ragnar Söderberg Foundation, and The Swedish Cancer Foundation.


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© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  1. 1.Department of Anatomy, Physiology and BiochemistrySwedish University of Agricultural SciencesUppsalaSweden

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