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Proteoglycans pp 201-217 | Cite as

Serglycin: The Master of the Mast Cell

  • Elin Rönnberg
  • Gunnar PejlerEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 836)

Abstract

Serglycin is a proteoglycan composed of a relatively small (∼17 kDa) core protein to which sulfated glycosaminoglycans of either heparin, heparan sulfate or chondroitin sulfate types are attached. Serglycin is expressed in many cell types, including in particular cells of hematopoietic origin. To study the function of serglycin, we have used a serglycin knockout mouse strain. A striking finding was that the mast cell population was severely affected by the absence of serglycin, as evidenced by distorted granule morphology and defective staining with cationic dyes. Moreover, the absence of serglycin caused a dramatic effect on the ability of mast cells to store a number of granule compounds, including several mast cell-specific proteases as well as biogenic amines. Hence, serglycin has a major function in maintaining mast cell secretory granule homeostasis.

Key words

Serglycin Proteoglycans Mast cells Granules Secretion 

Abbreviations

MC

Mast cell

BMMC

Bone marrow-derived mast cell

mMCP

Mouse mast cell protease

CS

Chondroitin sulfate

PG

Proteoglycan

GAG

Glycosaminoglycan

SCF

Stem cell factor

MC-CPA

Mast cell carboxypeptidase A

HPRT

Hypoxanthine guanine phosphoribosyl transferase

NDST

N-deacetylase/N-sulfotransferase

GalNAc

N-acetyl-galactosamine

C4ST

Chondroitin 4-sulfotransferase

GalNAc4S6ST

GalNAc(4S)-6-O-sulfotransferase

C6ST

Chondroitin 6-sulfotransferase

GlcUA

Glucuronic acid

IdoUA

Iduronic acid

WT

Wild type

Notes

Acknowledgments

The authors of this chapter receive support from The Swedish Research Council, Formas, King Gustaf V’s 80-year Anniversary Fund, Torsten and Ragnar Söderberg Foundation, and The Swedish Cancer Foundation.

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  1. 1.Department of Anatomy, Physiology and BiochemistrySwedish University of Agricultural SciencesUppsalaSweden

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