Protein NMR Techniques pp 197-218

Part of the Methods in Molecular Biology book series (MIMB, volume 831)

NMR Studies of Protein–RNA Interactions

Protocol

Abstract

This chapter describes the preparation of NMR quantities of RNA purified to single-nucleotide resolution for protein–RNA interaction studies. The protocol is easily modified to make nucleotide-specific isotopically labeled RNAs or uniformly labeled RNA fragments for ligation to generate segmentally labeled RNAs.

Key words

In vitro transcription Single-nucleotide resolution RNA synthesis Protein–RNA interactions RNA purification Isotopic labeling 

Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Carla A. Theimer
    • 1
  • Nakesha L. Smith
    • 1
  • May Khanna
    • 2
  1. 1.Department of ChemistryUniversity at Albany SUNYAlbanyUSA
  2. 2.Department of Biochemistry and Molecular BiologyIndiana University School of MedicineIndianapolisUSA

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