Chromatin Remodeling pp 103-113

Part of the Methods in Molecular Biology book series (MIMB, volume 833)

Generation of DNA Circles in Yeast by Inducible Site-Specific Recombination

Protocol

Abstract

Site-specific recombinases have been harnessed for a variety of genetic manipulations involving the gain, loss, or rearrangement of genomic DNA in a variety of organisms. The enzymes have been further exploited in the model eukaryote Saccharomyces cerevisiae for mechanistic studies involving chromosomal context. In these cases, a chromosomal element of interest is converted into a DNA circle within living cells, thereby uncoupling the element from neighboring regulatory sequences, obligatory chromosomal events, and other context-dependent effects that could alter or mask intrinsic functions of the element. In this chapter, I discuss general considerations in using site-specific recombination to create DNA circles in yeast and the specific application of the R recombinase.

Key words

Site-specific recombination R Recombinase RS site DNA circle Saccharomyces cerevisiae 

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  1. 1.Department of Pharmacology, Robert Wood Johnson Medical SchoolUniversity of Medicine and Dentistry of New JerseyPiscatawayUSA
  2. 2.The Cancer Institute of New JerseyNew BrunswickUSA

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