Analysis of Changes in Nucleosome Conformation Using Fluorescence Resonance Energy Transfer

  • Tina Shahian
  • Geeta J. Narlikar
Part of the Methods in Molecular Biology book series (MIMB, volume 833)


ATP-dependent nucleosome-remodeling motors use the energy of ATP to alter the accessibility of the underlying DNA. Understanding how these motors alter nucleosome structure can be aided by following changes in histone–DNA contacts in real time. Here, we describe a fluorescence resonance energy transfer-based approach that enables visualization of such changes.

Key words

Histone ATP-dependent chromatin remodeling ACF SNF2h 


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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  1. 1.Department of Biochemistry and BiophysicsUniversity of CaliforniaSan FranciscoUSA

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