Chromatin Remodeling pp 237-253

Part of the Methods in Molecular Biology book series (MIMB, volume 833)

Reconstitution of Modified Chromatin Templates for In Vitro Functional Assays

Protocol

Abstract

To study the functions of histone modifications in the context of chromatin, it is necessary to be able to prepare nucleosomal templates that carry specific posttranslational modifications in a defined biochemical system. Here, we describe two sets of protocols for reconstituting designer nucleosomes that contain specifically modified histones. The resulting nucleosomes are suitable for electromobility shift assays, chromatin remodeling assays, and other functional and structural studies.

Key words

Reconstitution In vitro Chromatin Histone modification Nucleosome 

Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  1. 1.Department of Molecular BiologyUT Southwestern Medical CenterDallasUSA
  2. 2.Department of Biochemistry and Molecular BiologyUniversity of Ulsan College of MedicineSeoulSouth Korea

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