Ubiquitin Family Modifiers and the Proteasome pp 163-171

Part of the Methods in Molecular Biology book series (MIMB, volume 832)

Role of UbL Family Modifiers and Their Binding Proteins in Cell Signaling

  • Sjoerd J. L. van Wijk
  • Magda Bienko
  • Ivan Dikic
Protocol

Abstract

The versatile function of ubiquitin (Ub) is powerfully illustrated by its appearance in multiple forms and shapes, like polymeric ubiquitin chains. These chains, when recognized by specific ubiquitin-binding domains (UBDs), give rise to extraordinary complex signaling networks that regulate virtually every cellular function. At the heart of our understanding of this complexity is the evolution and adaptation of technologies and methods to analyze ubiquitin biochemistry, e.g., covalent Ub–substrate conjugates as well as transient Ub–UBD interactions. Here, we describe seminal developments in those methodologies that have paved the way to our understanding of the diversity of Ub signals as well as their recognition and interpretation by UBD-containing proteins.

Key words

Polyubiquitin chains Ubiquitin-binding domains Linkage-specific antibodies Mass spectrometry SILAC AQUA 

Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Sjoerd J. L. van Wijk
    • 1
  • Magda Bienko
    • 1
    • 2
  • Ivan Dikic
    • 1
  1. 1.Frankfurt Institute for Molecular Life Sciences and Institute of Biochemistry IIGoethe University School of MedicineFrankfurt (Main)Germany
  2. 2.Departments of Physics and of BiologyMassachusetts Institute of TechnologyCambridgeUSA

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