Purification and Culture of Fetal Mouse Hepatoblasts that Are Precursors of Mature Hepatocytes and Biliary Epithelial Cells

Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 826)

Abstract

To investigate cell–cell interactions during mammalian liver development, it is essential to separate hepatoblasts (fetal liver progenitor cells) from nonparenchymal cells, including stellate cells, endothelial cells, and hemopoietic cells. Various factors, which may be produced by nonparenchymal cells, could be assayed for their effects on the growth and maturation of separated hepatoblasts. The protocol using immunomagnetic beads coated with anti-mouse E-cadherin antibody is described for efficient isolation of hepatoblasts from cell suspensions of fetal mouse livers. The purity and recovery rate are larger than 95% and approximately 30%, respectively. The protocol may be useful for various studies focusing on the fetal liver progenitor cells.

Key words

Hepatoblasts Hepatic progenitor cells E-cadherin Nonparenchymal cells Cell–cell interaction MACS 

Notes

Acknowledgments

This work was supported in part by Special Coordination Funds for Promoting Science and Technology from the Ministry of Education, Culture, Sports, Science, and Technology, the Japanese Government.

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  1. 1.Department of Biology, Faculty of ScienceShizuoka UniversityShizuokaJapan

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