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SNaPshot® Minisequencing Analysis of Multiple Ancestry-Informative Y-SNPs Using Capillary Electrophoresis

  • Maria Geppert
  • Lutz Roewer
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 830)

Abstract

This protocol describes a strategy for analyzing phylogenetic Y-SNPs in a hierarchical multiplex assay by utilizing the SNaPshot® Multiplex System. Step by step, the protocol assists in the appropriate selection of SNPs, the primer design, the set up of PCR/SBE reactions as well as in the analysis of the results. Furthermore, a forensic approach is highlighted, in which the most probable ancestry of an unknown male DNA is inferred by the geographical distribution of the assigned Y-SNP haplogroup.

Key words

Multiplex PCR Capillary electrophoresis SNaPshot® Minisequencing Y-SNP AIM 

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  1. 1.Department of Forensic Genetics, Institute of Legal Medicine and Forensic SciencesCharité-UniversitätsmedizinBerlinGermany

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