Bone Research Protocols pp 103-117

Part of the Methods in Molecular Biology book series (MIMB, volume 816)

Rodent Osteoclast Cultures

Protocol

Abstract

This chapter describes quantitative methods for isolating and culturing rodent osteoclasts on dentine, a bone-like, resorbable substrate. These techniques generate relatively large numbers of osteoclasts and allow the key processes of osteoclast formation and activation to be studied independently. A special focus will be on the role of extracellular pH, a critical factor in the control of osteoclast function.

Key words

Osteoclast Resorption pH Bone Dentine 

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  1. 1.Department of Cell and Developmental BiologyUniversity College LondonLondonUK

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