Fluorescence Measurement of Mitochondrial Membrane Potential Changes in Cultured Cells

Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 810)

Abstract

The mitochondrial membrane potential is the dominant component of the proton-motive force that is the potential term in the proton circuit linking electron transport to ATP synthesis and other energy-dependent mitochondrial processes. Cationic fluorescent probes have been used for many years to detect gross qualitative changes in mitochondrial membrane potentials in intact cell culture. In this chapter, I describe how these fluorescence signals may be used to obtain a semiquantitative measure of changes in mitochondrial membrane potential.

Key words

Mitochondria Membrane potential Neuron Glutamate Proton-motive force Proton electrochemical potential Tetramethylrhodamine methyl ester Rhodamine 123 JC-1 

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  1. 1.Buck Institute for Research on AgingNovatoUSA

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