Fluorescence Measurement of Mitochondrial Membrane Potential Changes in Cultured Cells
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The mitochondrial membrane potential is the dominant component of the proton-motive force that is the potential term in the proton circuit linking electron transport to ATP synthesis and other energy-dependent mitochondrial processes. Cationic fluorescent probes have been used for many years to detect gross qualitative changes in mitochondrial membrane potentials in intact cell culture. In this chapter, I describe how these fluorescence signals may be used to obtain a semiquantitative measure of changes in mitochondrial membrane potential.
Key wordsMitochondria Membrane potential Neuron Glutamate Proton-motive force Proton electrochemical potential Tetramethylrhodamine methyl ester Rhodamine 123 JC-1
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