Fluorescence Measurement of Mitochondrial Membrane Potential Changes in Cultured Cells

  • David G. NichollsEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 810)


The mitochondrial membrane potential is the dominant component of the proton-motive force that is the potential term in the proton circuit linking electron transport to ATP synthesis and other energy-dependent mitochondrial processes. Cationic fluorescent probes have been used for many years to detect gross qualitative changes in mitochondrial membrane potentials in intact cell culture. In this chapter, I describe how these fluorescence signals may be used to obtain a semiquantitative measure of changes in mitochondrial membrane potential.

Key words

Mitochondria Membrane potential Neuron Glutamate Proton-motive force Proton electrochemical potential Tetramethylrhodamine methyl ester Rhodamine 123 JC-1 


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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  1. 1.Buck Institute for Research on AgingNovatoUSA

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