Ribosome Display and Related Technologies

Volume 805 of the series Methods in Molecular Biology pp 393-401


Optimization of CAT-354, a Therapeutic Antibody Directed Against Interleukin-13, Using Ribosome Display

  • George ThomAffiliated withDepartment of Antibody Discovery and Protein Engineering, MedImmune
  • , Ralph MinterAffiliated withDepartment of Antibody Discovery and Protein Engineering, MedImmune Email author 

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In this case study, we describe the use of in vitro protein evolution with ribosome display to improve the potency of a human interleukin-13-neutralising antibody by a factor of over 200-fold and derive a therapeutic candidate, CAT-354, for the treatment of asthma. A combination of directed and random mutagenesis enabled the identification of highly potent neutralising antibodies and highlighted the advantage of the ribosome display protein evolution approach in identifying beneficial mutations across the entire sequence space. This chapter describes in detail the process followed to achieve a successful in vitro affinity maturation outcome using ribosome display technology.

Key words

Ribosome display Interleukin-13 Protein evolution scFv Antibody