Ribosome Display: A Perspective

Protocol
First Online:
Part of the Methods in Molecular Biology book series (MIMB, volume 805)

Abstract

Ribosome display is an in vitro evolution technology for proteins. It is based on in vitro translation, but prevents the newly synthesized protein and the mRNA encoding it from leaving the ribosome. It thereby couples phenotype and genotype. Since no cells need to be transformed, very large libraries can be used directly in selections, and the in vitro amplification provides a very convenient integration of random mutagenesis that can be incorporated into the procedure. This review highlights concepts, mechanisms, and different variations of ribosome display and compares it to related methods. Applications of ribosome display are summarized, e.g., the directed evolution of proteins for higher binding affinity, for higher stability or other improved biophysical parameters and enzymatic properties. Ribosome display has developed into a robust technology used in academia and industry alike, and it has made the cell-free Darwinian evolution of proteins over multiple generations a reality.

Key words:

Directed evolution Cell-free translation Ribosome display Protein engineering Antibody engineering DARPins Designed ankyrin repeat proteins Affinity maturation 
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Notes

Acknowledgments

My sincere thanks go to the many coworkers mentioned in the references who have developed and continuously improved the ribosome display technology over the years. I am grateful to Drs. Birgit Dreier, Oliver Scholz, Erik Sedlak and to Johannes Schilling for critically reading the manuscript.

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  1. 1.Department of BiochemistryUniversity of ZurichZurichSwitzerland

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