Transcriptional Regulation pp 465-472

Part of the Methods in Molecular Biology book series (MIMB, volume 809)

Affi nity Purifi cation of MLL3/MLL4 Histone H3K4 Methyltransferase Complex

Abstract

Methylation on histone H3 lysine 4 (H3K4) correlates with actively transcribed genes. In mammalian cells, there exist multiple Set1-like histone H3K4 methyltransferase complexes, which have overlapping but distinct subunit compositions. Developing methods to isolate each of these histone H3K4 methyltransferase complexes would help understand the molecular mechanisms by which histone H3K4 methylation regulates mammalian gene expression. In this chapter, we provide a one-step affinity purification protocol on isolation of the MLL3/MLL4 histone H3K4 methyltransferase complex using FLAG-tagged PA1, a unique subunit of the MLL3/MLL4 complex.

Key words

Histone H3K4 methyltransferase complex MLL3 MLL4 PA1 

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  1. 1.Nuclear Receptor Biology Section, NIDDK, NIHBethesdaUSA

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