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Introduction to Cell Culture

  • Christina Philippeos
  • Robin D. Hughes
  • Anil Dhawan
  • Ragai R. MitryEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 806)

Abstract

The basics of cell culture as applied to human cells are discussed. Biosafety when working with human tissue, which is often pathogenic, is important. The requirements for a tissue culture laboratory are described, particularly the range of equipment needed to carry out cell isolation, purification, and culture. Steps must be taken to maintain aseptic conditions to prevent contamination of cultures with micro-organisms. Basic cell-handling techniques are discussed, including choice of media, primary culture, and cryopreservation of cells so they can be stored for future use. Common assays which are used to determine cell viability and activity are considered.

Key words

Biosafety Tissue culture laboratory Aseptic technique Cell viability 

References

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Christina Philippeos
    • 1
  • Robin D. Hughes
    • 1
  • Anil Dhawan
    • 1
  • Ragai R. Mitry
    • 2
    Email author
  1. 1.Institute of Liver StudiesKing’s College HospitalLondonUK
  2. 2.Institute of Liver StudiesKing’s College London School of MedicineLondonUK

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