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Recombinant Protein Expression in the Baculovirus-Infected Insect Cell System

  • Tamar Unger
  • Yoav Peleg
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 800)

Abstract

Over the last two decades, the use of eukaryotic cells for expression of recombinant proteins has become the preferred choice for many applications. This is primarily the case when posttranslational modifications and correct disulfide-bond formation are necessary for protein folding and activity. Among the eukaryotic expression systems, the baculovirus-infected insect cell platform has gained particular attention, resulting in the development and implementation of multiple strategies for protein expression. Here, we present baculovirus-infected insect cells as an efficient expression system for eukaryotic proteins. We demonstrate a simplified and a shortened procedure for recombinant virus production that is sufficient for large-scale production of proteins in insect cells.

Key words

Baculovirus Insect cells Protein expression and production Secreted proteins Intracellular proteins 

Notes

Acknowledgments

We thank Prof. J. Sussman, Prof. I. Silman, Prof. G. Schreiber and Prof. Yigal Burstein for their continuous support throughout the study. This research was supported by the European Commission Sixth Framework Research and Technological Development Program ‘SPINE2-COMPLEXES’ Project, under contract No. 031220; a grant from the Israel Ministry of Science, Culture, and Sport to the Israel Structural Proteomics Center; the Divadol Foundation; and the Neuman Foundation.

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  1. 1.Israel Structural Proteomics Center, Faculty of BiochemistryWeizmann Institute of ScienceRehovotIsrael

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