Myogenesis pp 127-152 | Cite as

Analysis of Skeletal Muscle Development in Drosophila

  • Ginny R. Morriss
  • Anton L. Bryantsev
  • Maria Chechenova
  • Elisa M. LaBeau
  • TyAnna L. Lovato
  • Kathryn M. Ryan
  • Richard M. Cripps
Protocol
First Online:
Part of the Methods in Molecular Biology book series (MIMB, volume 798)

Abstract

The Drosophila system has been invaluable in providing important insights into mesoderm specification, muscle specification, myoblast fusion, muscle differentiation, and myofibril assembly. Here, we present a series of Drosophila protocols that enable the researcher to visualize muscle precursors and differentiated muscles, at all stages of development. In doing so, we also highlight the variety of techniques that are used to create these findings. These protocols are directly used for the Drosophila system, and are provided with explanatory detail to enable the researcher to apply them to other systems.

Key words

Drosophila Muscle Embryo Larva Pupa Adult Founder cell Myoblast Development Myogenesis Method Protocol Staining Hybridization 
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Notes

Acknowledgments

Research in the Cripps laboratory is funded by grants from the National Institutes of Health, the American Heart Association, the March of Dimes Birth Defects Foundation, and the Muscular Dystrophy Association. GRM and EML were supported by the NIGMS/IMSD award GM060201. EML is supported by a predoctoral fellowship from the AHA.

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Ginny R. Morriss
    • 1
  • Anton L. Bryantsev
    • 1
  • Maria Chechenova
    • 1
  • Elisa M. LaBeau
    • 1
  • TyAnna L. Lovato
    • 1
  • Kathryn M. Ryan
    • 1
  • Richard M. Cripps
    • 1
  1. 1.Department of BiologyUniversity of New MexicoAlbuquerqueUSA

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