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Unnatural Amino Acids pp 241–249Cite as

Site-Specific Modification of Proteins by the Staudinger-Phosphite Reaction

Part of the Methods in Molecular Biology book series (MIMB,volume 794)

Abstract

Chemoselective reactions are important tools for the modification of peptides and proteins. Thereby the modification is desired to be site specific and bioorthogonal. Here we describe the site-specific modification of azido-proteins via a Staudinger-type phosphite ligation. The reaction was carried out in aqueous system on proteins containing p-azido-phenylalanine in a single position introduced by the amber codon technique. A selective introduction of branched polyethylene scaffolds can be achieved with the application of the methodology reported herein.

Key words

  • Site-specific protein modification
  • Azido-proteins
  • Bioorthogonal
  • Staudinger-phosphite ligation
  • Protein PEGylation

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  • DOI: 10.1007/978-1-61779-331-8_15
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Acknowledgments

The authors acknowledge financial support from the German Science Foundation (DFG) within the Emmy-Noether program (HA 4468/2-1), the SFB 765, the Fonds der Chemischen Industrie (FCI) and the Böhringer-Ingelheim Foundation (“Plus 3-Perspektiven Programm”).

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Authors and Affiliations

  1. Institut für Chemie und Biochemie, Freie Universität Berlin, Berlin, Germany

    Paul Majkut, Verena Böhrsch, Remigiusz Serwa & Christian P. R. Hackenberger

  2. Labor fur Biochemie, Beuth-Hochschule für Technik, Berlin, Germany

    Verena Böhrsch

  3. RiNA GmbH, Berlin, Germany

    Michael Gerrits

Authors
  1. Paul Majkut
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  2. Verena Böhrsch
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  3. Remigiusz Serwa
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  4. Michael Gerrits
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  5. Christian P. R. Hackenberger
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Corresponding author

Correspondence to Christian P. R. Hackenberger .

Editor information

Editors and Affiliations

  1. Dipto. Biotecnologie e di Scienze Mol, Molecolari (DBSM), Università degli Studi dell'Insubria, Via J.H. Dunant 3, Varese, 21100, Italy

    Loredano Pollegioni

  2. Dipto. Chimica, Politecnico di Milano, Via Mancinelli 7, Milano, 20131, Italy

    Stefano Servi

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Majkut, P., Böhrsch, V., Serwa, R., Gerrits, M., Hackenberger, C.P.R. (2012). Site-Specific Modification of Proteins by the Staudinger-Phosphite Reaction. In: Pollegioni, L., Servi, S. (eds) Unnatural Amino Acids. Methods in Molecular Biology, vol 794. Humana Press. https://doi.org/10.1007/978-1-61779-331-8_15

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  • DOI: https://doi.org/10.1007/978-1-61779-331-8_15

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