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Nanoproteomics pp 277-285 | Cite as

Functionalized Soluble Nanopolymers for Phosphoproteome Analysis

  • Anton Iliuk
  • Keerthi Jayasundera
  • Rachel Schluttenhofer
  • W. Andy TaoEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 790)

Abstract

Protein phosphorylation plays a critical role in the regulation of many cellular functions. Phosphoproteomic analyses facilitate an in-depth understanding of such phosphorylation-dependent signaling networks. The use of mass spectrometry in phosphoproteomics has been especially successful, but the approach largely depends on an efficient method to enrich phosphopeptides from complex mixtures. We have developed a novel, effective soluble nanopolymer-based phosphopeptide enrichment technique, termed PolyMAC (polymer-based metal ion affinity capture). The homogenous, hyperfunctional nature of PolyMAC reagent makes it a more competent choice for highly efficient phosphopeptide binding and isolation, which was demonstrated through several applications with simple protein mixture and complex cell extract.

Key words

PolyMAC Phosphoproteomics Phosphopeptide enrichment Dendrimer Nanopolymer Titania 

Notes

Acknowledgments

The authors gratefully acknowledge that this work has been funded in part by an NSF CAREER award, a 3M general fund, and National Institutes of Health grants 1S10RR025044 (high-end instrumentation grant) and R01GM088317.

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Anton Iliuk
    • 1
  • Keerthi Jayasundera
    • 1
  • Rachel Schluttenhofer
    • 1
  • W. Andy Tao
    • 1
    Email author
  1. 1.Departments of Biochemistry, Chemistry, and Medicinal Chemistry, and Molecular Pharmacology, Purdue Center for Cancer ResearchPurdue UniversityWest LafayetteUSA

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