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Methylation-Specific PCR

  • Ja-Lok Ku
  • You-Kyung Jeon
  • Jae-Gahb Park
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 791)

Abstract

DNA methylation patterns in CpG-rich regions of promoter, CpG islands, are concerned in regulation of gene expression in mammalian cells. Excessive methylation of CpG dinucleotides in promoter represses the gene expression. In cancer, especially, gene silencing is occurred through aberrant methylation in promoter of tumor suppressor genes. Methylation-specific PCR (MSP) is a method for analysis of DNA methylation patterns in CpG islands. For performing MSP, DNA is modified by and PCR performed with two primer pairs, which are detectable methylated and unmethylated DNA, respectively. MSP is a rapid measure for assession of the methylation status in CpG island.

Key words

Methylation-specific PCR CpG islands Bisulfite conversion Methylation and unmethylation primer MSP primer design 

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  1. 1.Laboratory of Cell Biology, Korean Cell Line Bank, Cancer Research InstituteSeoul National University College of MedicineSeoulSouth Korea

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