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Studying RNA–Protein Interactions In Vivo By RNA Immunoprecipitation

  • Luke A. Selth
  • Pierre Close
  • Jesper Q. Svejstrup
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 791)

Abstract

The crucial roles played by RNA-binding proteins in all aspects of RNA metabolism, particularly in the regulation of transcription, have become increasingly evident. Moreover, other factors that do not directly interact with RNA molecules can nevertheless function proximally to RNA polymerases and have significant effects on gene expression. RNA immunoprecipitation (RIP) is a powerful technique used to detect direct and indirect interactions between individual proteins and specific RNA molecules in vivo. Here, we describe RIP methods for both yeast and mammalian cells.

Key words

RNA immunoprecipitation RNA-binding protein Transcription factor mRNA Chromatin immunoprecipitation Gene expression 

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Luke A. Selth
    • 1
  • Pierre Close
    • 2
  • Jesper Q. Svejstrup
    • 3
  1. 1.Dame Roma Mitchell Cancer Research LaboratoriesThe University of AdelaideAdelaideAustralia
  2. 2.Unit of Medical Chemistry, GIGA-Signal TransductionUniversity of LiegeLiegeBelgium
  3. 3.Mechanisms of Gene Transcription Laboratory,Clare Hall LaboratoriesCancer Research UK London Research InstituteHertfordshireUK

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