A High-throughput Gateway-Compatible Yeast One-Hybrid Screen to Detect Protein–DNA Interactions

Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 786)

Abstract

In recent years, new techniques have spurred the discovery of cis-regulatory DNA elements. These stretches of noncoding DNA contain combinations of recognition sites to which transcription factors (TFs) bind, and in doing so, these TFs can activate or repress transcription. These protein–DNA interactions form the core of gene regulatory networks (GRNs) that are responsible for the differential gene expression that allow diversification of cell types, developmental programs, and responses to the environment. The yeast one-hybrid system is a genetic assay to identify direct binding of proteins to DNA elements of interest and is, therefore, instrumental in uncovering these GRNs.

Key words

Yeast one-hybrid Transcription factor Protein–DNA interaction Gateway Regulatory element Gene regulatory element 

Notes

Acknowledgments

This work was supported by funds from the Swiss National Science Foundation, by Systems X, by a Marie Curie International Reintegration Grant (BD) from the Seventh Research Framework Programme, and by Institutional support from the Ecole Polytechnique Fédérale de Lausanne (EPFL).

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Korneel Hens
    • 1
  • Jean-Daniel Feuz
    • 1
  • Bart Deplancke
    • 1
  1. 1.Laboratory of Systems Biology and GeneticsÉcole Polytechnique Fédérale de Lausanne (EPFL)LausanneSwitzerland

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