Gene Regulatory Networks pp 21-50

Part of the Methods in Molecular Biology book series (MIMB, volume 786) | Cite as

Expression Pattern Analysis of Regulatory Transcription Factors in Caenorhabditis elegans

Protocol

Abstract

Expression pattern data are fundamental to understanding transcriptional regulatory networks and the biological significance of such networks. For Caenorhabditis elegans, expression pattern analysis of transcription factor genes, with cellular resolution, typically involves generation of transcription factor gene/reporter gene fusions. This is followed by the creation of C. elegans strains transgenic for, and determination of expression patterns driven by, these fusions. Physiologically relevant regulatory relationships between transcription factors are both inferred from their expression patterns, in combination with protein–DNA interaction data, and evidenced from alterations of expression patterns when networks are disturbed.

Key words

Transcription factor Gateway Recombineering Reporters Caenorhabditis elegans 

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  1. 1.Faculty of Biological Sciences, Institute of Integrative and Comparative BiologyThe University of LeedsLeedsUK

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