CAGE (Cap Analysis of Gene Expression): A Protocol for the Detection of Promoter and Transcriptional Networks

  • Hazuki Takahashi
  • Sachi Kato
  • Mitsuyoshi Murata
  • Piero Carninci
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 786)

Abstract

We provide here a protocol for the preparation of cap-analysis gene expression (CAGE) libraries, which allows for measuring the expression of eukaryotic capped RNAs and simultaneously map the promoter regions. The presented protocol simplifies the previously published ones and moreover produces tags that are 27 nucleotides long, which facilitates mapping to the genome. The protocol takes less than 5 days to complete and presents a notable improvement compared to previously published versions.

Key words

Cap-analysis gene expression RNAseq Transcriptome Sequencing RNA 

Notes

Acknowledgments

This work was founded by a Research Grant for RIKEN Omics Science Center from MEXT and by the National Human Genome Research Institute grants U54 HG004557. We thank all the ­colleagues at the OSC for the precious feedbacks during the development of the methodology.

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Hazuki Takahashi
    • 1
  • Sachi Kato
    • 1
  • Mitsuyoshi Murata
    • 1
  • Piero Carninci
    • 1
  1. 1.Riken Omics Science CenterRiken Yokohama InstituteYokohamaJapan

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