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High-Throughput Studies of Protein Glycoforms Using Antibody–Lectin Sandwich Arrays

  • Brian B. HaabEmail author
  • Tingting Yue
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 785)

Abstract

The antibody–lectin sandwich arrays (ALSA) is a powerful new tool for glycoproteomics research. ALSA enables precise measurements of the glycosylation states of multiple proteins captured directly from biological samples. The platform can be used in a high-throughput mode with low sample consumption, making it well suited to biomarker research exploring glycan alterations on specific proteins. This article provides detailed descriptions of the use of ALSA, with a particular focus on biomarker research. The preparation and selection of antibodies and lectins, the preparation and use of the arrays and samples, and special considerations for using the platform for biomarker research are covered.

Key words

Antibody arrays Antibody–lectin sandwich arrays Glycan profiling Lectin detection Serum biomarkers 

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  1. 1.Van Andel Research InstituteGrand RapidsUSA

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