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Assessment of Antibody Specificity Using Suspension Bead Arrays

  • Jochen M. Schwenk
  • Peter Nilsson
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 785)

Abstract

With the increasing collection of affinity reagents, their validation in terms of functionality and binding specificity becomes a challenge. To match this growing need, miniaturized and parallelized platforms have become available to corroborate the applicability for a broad range of binder scaffolds. Among the ­commonly used systems, planar microarrays have been a platform of choice for a long time but alternative systems are emerging, of which one is based on color-coded beads for the creation of arrays in suspension. The latter systems offer to perform a two-dimensional multiplexing by now analyzing up to 384 samples against up to 500 analytes in a single experiment. While the analyte parameter is flexible in terms of its composition, an extended screening can be facilitated without the need to set up a microarray production facility.

Key words

Suspension bead array Antibody specificity Cross-reactivity 

Notes

Acknowledgments

We are grateful to the entire staff within the Human Protein Atlas project for their tremendous efforts. This work is supported by the PRONOVA project (VINNOVA, Swedish Governmental Agency for Innovation Systems), and by grants from the Knut and Alice Wallenberg Foundation.

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  1. 1.SciLifeLab StockholmKTH – Royal Institute of TechnologyTomtebodavSweden

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