Fluorescence Microscopy of Nanochannel-Confined DNA

  • Fredrik Persson
  • Fredrik Westerlund
  • Jonas O. TegenfeldtEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 783)


Stretching of DNA in nanoscale confinement allows for direct visualization of the genetic contents of the DNA on the single DNA molecule level. DNA stretched in nanoscale confinement also allows for studies of DNA–protein interactions and DNA polymer physics in confined environments. This chapter describes the basic steps to fabricate the nanostructures, to perform the experiments, and to analyze the data.

Key words

DNA Nanochannels Single molecule Fluorescence 



We thank Camilla Freitag for valuable discussions and comments on the manuscript.

This work was supported by the European Community’s Seventh Framework Program [FP7/2007–2013] under grant agreement number [HEALTH-F4–2008–201418] entitled READNA, the Swedish Research Council under grants number 2007-584 and 2007-4454 and the Knut and Alice Wallenberg Foundation.


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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Fredrik Persson
    • 1
  • Fredrik Westerlund
    • 1
  • Jonas O. Tegenfeldt
    • 1
    • 2
    Email author
  1. 1.Department of PhysicsUniversity of GothenburgGothenburgSweden
  2. 2.Department of PhysicsLund UniversityLundSweden

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