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Analysis of Microtubule Assembly Kinetics Using Turbidimetry

  • Felicia Gaskin
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 777)

Abstract

Turbidity measurements are rapid and reliable methods to follow the effects of drugs, proteins, nucleotides, metals, and other factors on the assembly kinetics of tubulin into microtubules in vitro and have been in use since 1974. Improvements in spectrophotometers and software have resulted in the use of less protein, and more curves can be analyzed continuously and almost simultaneously.

Key words

Tubulin Assembly Turbidimetry Microtubules Kinetics 

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  1. 1.Department of Psychiatry and Neurobehavioral SciencesUniversity of Virginia School of MedicineCharlottesvilleUSA

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