Dynamic instability is a hallmark of the microtubule cytoskeleton. In order to regulate microtubule dynamics in vivo, a varied host of microtubule-associated proteins are mobilized to promote microtubule nucleation, growth, stabilization, catastrophe, depolymerization, rescue, and severing. To confer these various functions, cytoskeletal regulators have highly tuned affinities for tubulin, recognizing the unpolymerized αβ-tubulin heterodimer, dynamic microtubule lattice, stabilized microtubule lattice, or a combination therein. The protocols presented here probe αβ-tubulin and microtubule binding using gel filtration and cosedimentation, respectively.
Tubulin Tubulin-binding protein Microtubule-associated protein Gel filtration Microtubule cosedimentation assay Dynamic microtubules Taxol
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The authors would like to thank Dr. Stephen Rogers for advice. This work was supported by a Basil O’Connor Research Starter Grant from the March of Dimes.
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