Chloroplast Research in Arabidopsis pp 161-185

Part of the Methods in Molecular Biology book series (MIMB, volume 775) | Cite as

Chloroplast Phenomics: Systematic Phenotypic Screening of Chloroplast Protein Mutants in Arabidopsis

Abstract

As part of a project to analyze chloroplast functional networks systematically, we have subjected mutants in >3,200 nuclear genes predicted to encode chloroplast-targeted proteins in Arabidopsis thaliana (http://www.plastid.msu.edu) to parallel phenotypic assays. Detailed methods are presented for the various assays being used in this project to study chloroplast biology. These include morphological analysis of plants, chloroplasts, and seeds using controlled vocabulary. Metabolites synthesized in the chloroplast such as starch, amino acids, and fatty acids are analyzed in groups according to their chemical properties. As an indicator for the relative composition of seed storage oil and proteins, the carbon and nitrogen contents are determined by an elemental analyzer. The methods in this chapter describe how the assays are configured to run in relatively high throughput, maximizing data quality.

Key words

Amino acid Seed composition Chloroplast Chlorophyll fluorescence Fatty acid Genotyping Morphology Starch Systems biology Reverse genetics Phenomics 

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  1. 1.Department of Biological SciencesWestern Michigan UniversityKalamazooUSA
  2. 2.Department of Biochemistry and Molecular BiologyMichigan State UniversityEast LansingUSA
  3. 3.Department of Biochemistry and Molecular Biology, and Department of Plant BiologyMichigan State UniversityEast LansingUSA

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