Seed Dormancy pp 151-164 | Cite as

Isolation of Genetic Material from Arabidopsis Seeds

Part of the Methods in Molecular Biology book series (MIMB, volume 773)


Here, we describe a series of methods suitable for the reproducible and abundant isolation of total RNA, genomic DNA, and total protein from dry or imbibed Arabidopsis seeds. The resulting material is suitable for most standard molecular biology procedures.

Key words

Seed germination RNA isolation DNA isolation Protein isolation Protein transfer to membrane cDNA synthesis RT-PCR Microarray analysis Sodium bisulfite sequencing Radioactive in vivo labeling Immunopurification 


  1. 1.
    Vicient, C.M., Delseny, M. (1999) Isolation of total RNA from Arabidopsis thaliana seeds. Anal. Biochem. 268, 412–3.Google Scholar
  2. 2.
    Dellaporta, S.L., Wood, J., Hicks, J.B. (1983) A plant DNA minipreparation: version II. Plant Mol. Biol. Rep. 1, 19–21.Google Scholar
  3. 3.
    Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680–5.Google Scholar
  4. 4.
    Sambrook, J., Fritsch, E.F., Maniatis, T. (1989) Molecular Cloning, A Laboratory Manual. Cold Spring Harbor Laboratory Press.Google Scholar
  5. 5.
    Lopez-Molina, L., Mongrand, S., Chua, N.H. (2001) A postgermination developmental arrest checkpoint is mediated by abscisic acid and requires the ABI5 transcription factor in Arabidopsis. Proc. Natl. Acad. Sci. USA 98, 4782–7.Google Scholar
  6. 6.
    Ausubel, F.M., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A., Struhl, K. (1999) Short Protocols in Molecular Biology. New York: Wiley.Google Scholar
  7. 7.
    Perruc, E., Kinoshita, N., Lopez-Molina, L. (2007) The role of chromatin-remodeling factor PKL in balancing osmotic stress responses during Arabidopsis seed germination. Plant J. 52, 92736.Google Scholar
  8. 8.
    Kinoshita, N., Berr, A., Belin, C., Chappuis, R., Nishizawa, N.K., Lopez-Molina, L. (2010) Identification of growth insensitive to ABA3 (gia3), a recessive mutation affecting ABA Signaling for the control of early post-­germination growth in Arabidopsis thaliana. Plant Cell Physiol. 51, 239–51.Google Scholar

Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  1. 1.Département de Biologie VégétaleUniversité de GenèveGenèveSwitzerland
  2. 2.Département de Biologie VégétaleUniversité de GenèveGenevaSwitzerland

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