Seed Dormancy pp 151-164 | Cite as

Isolation of Genetic Material from Arabidopsis Seeds

Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 773)

Abstract

Here, we describe a series of methods suitable for the reproducible and abundant isolation of total RNA, genomic DNA, and total protein from dry or imbibed Arabidopsis seeds. The resulting material is suitable for most standard molecular biology procedures.

Key words

Seed germination RNA isolation DNA isolation Protein isolation Protein transfer to membrane cDNA synthesis RT-PCR Microarray analysis Sodium bisulfite sequencing Radioactive in vivo labeling Immunopurification 

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  1. 1.Département de Biologie VégétaleUniversité de GenèveGenèveSwitzerland
  2. 2.Département de Biologie VégétaleUniversité de GenèveGenevaSwitzerland

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