Reduced Representation Methods for Subgenomic Enrichment and Next-Generation Sequencing

Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 772)

Abstract

Several methods have been developed to enrich DNA for subsets of the genome prior to next-generation sequencing. These front-end enrichment strategies provide powerful and cost-effective tools for researchers interested in collecting large-scale genomic sequence data. In this review, I provide an overview of both general and targeted reduced representation enrichment strategies that are commonly used in tandem with next-generation sequencing. I focus on several key issues that are likely to be important when deciding which enrichment strategy is most appropriate for a given experiment. Overall, these techniques can enable the collection of large-scale genomic data in diverse species, providing a powerful tool for the study of evolutionary biology.

Key words

Reduced representation Sequence capture Targeted resequencing Ancient DNA Sample bar coding 

Notes

Acknowledgments

I thank Emily Hodges, Frank Albert, Martin Kircher, Adrian Briggs, Hernán Burbano, Gordon Luikart, and Matthias Meyer for many helpful conversations on NGS and targeted enrichment. Research contributing to this review was supported by an NSF international postdoctoral fellowship (OISE-0754461).

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  1. 1.Division of Biological SciencesUniversity of MontanaMissoulaUSA

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