Cell Migration pp 261-275 | Cite as

Measuring Inflammatory Cell Migration in the Zebrafish

  • Philip M. Elks
  • Catherine A. Loynes
  • Stephen A. Renshaw
Part of the Methods in Molecular Biology book series (MIMB, volume 769)


A key feature of inflammatory cells is the ability to migrate to a site of injury or infection quickly and efficiently. Infectious agents can then be taken up by these inflammatory cells, preventing established infection. Inflammatory cell migration is driven by a complex interaction between inflammatory cells and their environment. In order to maintain health, inflammation needs to resolve, allowing the surrounding tissues to recover and heal. These processes are not fully understood and have been difficult to study in cell culture due to the complex interactions between cell types. We therefore use a range of techniques in near-transparent zebrafish (Danio rerio) larvae to study these migration events in a whole-organism, in vivo model. Using a transgenic zebrafish line that specifically marks neutrophils with green fluorescent protein, Tg(mpx:GFP)i114, we are able to follow neutrophil behaviour at a single cell level. Using these methods, the cellular processes involved in all phases of inflammation can be studied and better understood.

Key words

Neutrophil Migration Inflammation Zebrafish In vivo 



SAR and CAL are funded by an MRC Senior Clinical Fellowship to SAR (reference number: G0701932). PME is funded by a project grant from the Wellcome Trust (reference number: WT082909MA). Microscopy studies in our laboratory are supported by a Wellcome Trust grant to the MBB/BMS Light microscopy facility (GR077544AIA), and the laboratory is supported by an MRC Centre grant (G0700091).


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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Philip M. Elks
  • Catherine A. Loynes
  • Stephen A. Renshaw
    • 1
  1. 1.MRC Centre for Developmental and Biomedical GeneticsThe University of SheffieldSheffieldUK

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