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Derivation of Oligodendrocyte Progenitor Cells from Human Embryonic Stem Cells

  • Jason Sharp
  • Maya Hatch
  • Gabriel Nistor
  • Hans KeirsteadEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 767)

Abstract

The directed differentiation of human pluripotent stem cells into specific, determined, and high-purity cell types can provide a means to study the cellular and molecular mechanisms of development and to generate cells for potential therapeutic applications. The ability to derive homogeneous cell populations obviates the need for transgene expression or cell sorting methods and can improve selection efficiency, lineage differentiation, cell viability, and clinical utility. Compared to undifferentiated pluripotent stem cells, high-purity cell phenotypes for clinical therapeutic strategies are expected to enhance engraftment, potentiate clinical efficacy, and decrease the risk of adverse effects such as dedifferentiation or teratoma formation. Clinical interest in the derivation of oligodendrocyte progenitor cells from pluripotent stem cells is based on research that demonstrates the effectiveness of progenitor cell transplants to improve outcomes after spinal cord injury. Here, we describe a protocol to generate oligodendroglial lineage-specific cells in high purity from human embryonic stem cells.

Key words

oligodendrocyte progenitor cells human embryonic stem cells high purity directed differentiation neurospheres 

References

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    Sharp J, Keirstead HS (2007). Therapeutic applications of oligodendrocyte precursors derived from human embryonic stem cells. Curr Opin Biotechnol. 18: 434–440.PubMedCrossRefGoogle Scholar
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    Nistor GI, Totoiu MO, Haque N, Carpenter MK, Keirstead HS (2005). Human embryonic stem cells differentiate into oligodendrocytes in high purity and remyelinate after spinal cord transplantation. Glia 49: 385–396.PubMedCrossRefGoogle Scholar
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    Izrael M, Zhang P, Kaufman R, Shinder V, Ella R, Amit M, Itskovitz-Eldor J, Chebath J, Revel M (2007). Human oligodendrocytes derived from embryonic stem cells: Effect of noggin on phenotypic differentiation in vitro and on myelination in vivo. Mol Cell Neurosci. 34: 310–323.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Jason Sharp
    • 1
  • Maya Hatch
    • 1
  • Gabriel Nistor
    • 1
  • Hans Keirstead
    • 2
    Email author
  1. 1.Keirstead Research Group, Anatomy and Neurobiology, School of MedicineUniversity of California, IrvineIrvineUSA
  2. 2.Reeve Irvine Research Center, Gillespie Neuroscience Research Facility, School of MedicineUniversity of California, IrvineIrvineUSA

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