Human Pluripotent Stem Cells pp 369-387 | Cite as
Episomal Transgene Expression in Pluripotent Stem Cells
Abstract
Herpes simplex type 1 (HSV-1) amplicon vectors possess a number of features that make them excellent vectors for the delivery of transgenes into stem cells. HSV-1 amplicon vectors are capable of efficiently transducing both dividing and nondividing cells and since the virus is quite large, 152 kb, it is of sufficient size to allow for incorporation of entire genomic DNA loci with native promoters. HSV-1 amplicon vectors can also be used to incorporate and deliver to cells a variety of sequences that allow extrachromosomal retention. These elements offer advantages over integrating vectors as they avoid transgene silencing and insertional mutagenesis. The construction of amplicon vectors carrying extrachromosomal retention elements, their packaging into HSV-1 viral particles, and the use of HSV-1 amplicons for stem cell transduction will be described.
Key words
HSV-1 amplicon iBAC Extrachromosomal vector Stem cells Gene expression vectorNotes
Acknowledgments
This work was supported by the Parkinson’s UK Monument Trust Discovery Award; the Friedreich’s Ataxia Research Alliance, Ataxia UK and the National Ataxia Foundation; the Medical Research Council and the Biotechnology and Biological Sciences Research Council. M.M.P.L. is an Ataxia UK Research Fellow, A.R.P. is a Medical Research Council student and S.A.C. is a Wellcome Trust Research Fellow.
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