Protocol

Human Pluripotent Stem Cells

Volume 767 of the series Methods in Molecular Biology pp 221-230

Date:

Flow Cytometric Analysis of Human Pluripotent Stem Cells

  • Mirabelle S. H. HoAffiliated withDepartment of Anatomy and Developmental Biology, Monash University
  • , Andrew FrygaAffiliated withFlow Core, Australian Stem Cell Centre and Monash University
  • , Andrew L. LaslettAffiliated withDepartment of Anatomy and Developmental Biology, Monash University Email author 

* Final gross prices may vary according to local VAT.

Get Access

Abstract

Human pluripotent stem cells, human embryonic stem cells and induced pluripotent stem cells, represent an exciting new era in regenerative medicine and drug discovery. However, prior to their clinical translation, there is a need to gain an in-depth understanding of human pluripotent stem cell biology by characterizing these potentially heterogeneous populations of cells. Flow cytometry provides a rapid and efficient approach with which to isolate, purify, and study the functional properties of defined pluripotent stem cell types.

Key words

human embryonic stem cells induced pluripotent stem cells flow cytometry