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Assessment of Intestinal Permeability in (Premature) Neonates by Sugar Absorption Tests

  • Willemijn E. Corpeleijn
  • Ruurd M. van Elburg
  • Ido P. Kema
  • Johannes B. van Goudoever
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 763)

Abstract

Infants born prematurely have an enhanced intestinal permeability compared to healthy term infants. This enhanced permeability might be a contributing factor in the development of Necrotising Enterocolitis. The assessment of intestinal permeability in premature neonates with sugar absorption tests has been proven to be safe and of minimal burden to the infant. After enteral administration of a test solution containing lactulose and mannitol, the excretion of these sugars is measured in urine, and the ratio is calculated. The lactulose and mannitol concentrations in urine can be measured by the use of a gas chromatograph after pre-purification and derivatisation of the sample. Non-invasive assessment of intestinal permeability can be useful in monitoring the effects of experimental (nutritional) therapy.

Key words

Intestinal permeability Premature neonate Lactulose Mannitol Sugar absorption test Gas chromatography 

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Willemijn E. Corpeleijn
    • 1
  • Ruurd M. van Elburg
    • 2
  • Ido P. Kema
    • 3
  • Johannes B. van Goudoever
    • 1
  1. 1.Division of NeonatologyErasmus MC – Sophia Children’s HospitalRotterdamThe Netherlands
  2. 2.Department of Pediatrics, Division of NeonatologyVrije Universiteit Medical CenterAmsterdamThe Netherlands
  3. 3.Department of Laboratory Medicine, Division of Clinical Chemistry and HematologyUniversity Medical Center GroningenGroningenThe Netherlands

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