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Claudins pp 27-41 | Cite as

Electrophysiological Characterization of Claudin Ion Permeability Using Stably Transfected Epithelial Cell Lines

  • Alan S. L. YuEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 762)

Abstract

Claudins are tight junction membrane proteins that act as paracellular pores and barriers and regulate epithelial permeability to small ions. A key step in understanding the function of any claudin isoform is the in vitro measurement of its ion permeability and selectivity. Herein, we describe methods to generate clonal lines with stable inducible overexpression of claudins in Madin–Darby canine kidney epithelial cells, measure conductance and diffusion potentials in Ussing chambers, correct for liquid junction potentials, and derive quantitatively accurate values for individual ion permeabilities.

Key words

Claudin Tight junction Paracellular Diffusion potential Conductance Ion permeability Junction potential 

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  1. 1.Division of Nephrology and Hypertension and the Kidney InstituteUniversity of Kansas Medical CenterKansas CityUSA

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