Polyadenylation State Microarray (PASTA) Analysis

  • Traude H. Beilharz
  • Thomas Preiss
Part of the Methods in Molecular Biology book series (MIMB, volume 759)


Nearly all eukaryotic mRNAs terminate in a poly(A) tail that serves important roles in mRNA utilization. In the cytoplasm, the poly(A) tail promotes both mRNA stability and translation, and these functions are frequently regulated through changes in tail length. To identify the scope of poly(A) tail length control in a transcriptome, we developed the polyadenylation state microarray (PASTA) method. It involves the purification of mRNA based on poly(A) tail length using thermal elution from poly(U) sepharose, followed by microarray analysis of the resulting fractions. In this chapter we detail our PASTA approach and describe some methods for bulk and mRNA-specific poly(A) tail length measurements of use to monitor the procedure and independently verify the microarray data.

Key words

Poly(A) tail polyadenylation deadenylation post-transcriptional regulation translational control mRNA stability polysome microarray transcriptome proteome 



We thank Jürg Bähler and Daniel Lackner for their input to PASTA method development and application. This work was supported by the Victor Chang Cardiac Research Institute, the Australian Research Council, the National Health and Medical Research Council of Australia, and the Sylvia and Charles Viertel Charitable Foundation.


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Copyright information

© Humana Press 2011

Authors and Affiliations

  1. 1.Department of Biochemistry and Molecular BiologyMonash UniversityClaytonAustralia
  2. 2.Genome Biology DepartmentThe John Curtin School of Medical Research, The Australian National UniversityActon (Canberra)Australia

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