Microglia Cell Culture: A Primer for the Novice

  • Anke Witting
  • Thomas MöllerEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 758)


Microglial cells are the resident immune cells of the central nervous system. Progress in the recent decade has clearly established that microglial cells participate or even actively drive neurological disease. Much of our current knowledge has been generated by investigating microglial cells in cell culture. The aim of this chapter is to give the uninitiated a basic and adaptable protocol for the culturing of microglial cells. We discuss the challenges of microglial cell culture and provide a collection of tips which reflect our 25+ years of collective experience.

Key words

Microglia Cell culture Medium Serum Growth factor M-CSF GM-CSF Transfection Endotoxin Cell yield 


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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  1. 1.Department of Neurology and Center for Neurogenetics and NeurotherapeuticsUniversity of WashingtonSeattleUSA

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