Protocol

In Vitro Neurotoxicology

Volume 758 of the series Methods in Molecular Biology pp 13-27

Date:

Primary Neurons in Culture and Neuronal Cell Lines for In Vitro Neurotoxicological Studies

  • Gennaro Giordano
  • , Lucio G. CostaAffiliated withDepartment of Environmental and Occupational Health Sciences, University of WashingtonDepartment of Human Anatomy, Pharmacology, and Forensic Science, University of Parma Medical School Email author 

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Abstract

Primary neuronal cultures and neuronal cell lines derived from rodents are widely used to study basic physiological properties of neurons, and represent a useful tool to study the potential neurotoxicity of chemicals. While short-term culturing of neurons can be a very straightforward process, long-term cultures of relatively pure neuronal populations require more effort. This chapter describes methods and protocols to isolate and culture primary neurons obtained from the rodent cerebellum (cerebellar granule cells), the hippocampus, and the striatum. Furthermore, culturing of rat pheochromocytoma (PC-12) cells is also described, as these cells represent a useful and widely used cell line for in vitro neurotoxicological studies.

Key words

Primary cultures Neuronal cell lines Cerebellar granule cells Hippocampal neurons Striatal neurons PC-12 cells