Bioconjugation Protocols pp 297-308

Part of the Methods in Molecular Biology book series (MIMB, volume 751) | Cite as

Lipid-Core-Peptide System for Self-Adjuvanting Synthetic Vaccine Delivery

Protocol

Abstract

Disadvantages of classical vaccines, such as the risk of an autoimmune reaction, might be overcome by using a subunit vaccine containing the minimal microbial components necessary to stimulate appropriate immune responses. However, vaccines based on minimal epitopes suffer from poor immunogenicity and require the use of an additional immunostimulant (adjuvant). Only a few adjuvants have been permitted for use with vaccines intended for human administration. We have developed several vaccine candidates based on a lipid-core-peptide (LCP) system. This system has self-adjuvanting properties, and it can be used for the delivery of a variety of epitopes to produce vaccine candidates against a targeted disease. The LCP system is easily assembled by simple stepwise Boc solid-phase peptide synthesis.

Key words

Lipoamino acids Synthetic vaccine Lipid-core-peptide Adjuvant Boc solid-phase peptide synthesis Multiple antigenic peptide 

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  1. 1.School of Chemistry and Molecular BiosciencesThe University of QueenslandSt. LuciaAustralia

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