Functional Genomics Assays to Study CFTR Traffic and ENaC Function
As several genomes have been sequenced, post-genomic approaches like transcriptomics and proteomics, identifying gene products differentially expressed in association with a given pathology, have held promise both of understanding the pathways associated with the respective disease and as a fast track to therapy. Notwithstanding, these approaches cannot distinguish genes and proteins with mere secondary pathological association from those primarily involved in the basic defect(s). New global strategies and tools identifying gene products responsible for the basic cellular defect(s) in CF pathophysiology currently being performed are presented here. These include high-content screens to determine proteins affecting function and trafficking of CFTR and ENaC.
Key wordsCystic fibrosis CFTR secretory traffic ENaC high-content screens siRNA functional genomics
This work is supported by TargetScreen2 (EU-FP6-LSH-2005-037365) grant. J.A. is recipient of Ph.D. fellowship SFRH/BD/29134/2006 (FCT, Portugal). The authors wish to thank Beate Neumann and Jutta Bulkescher (Advanced Light Microscopy Core Facility, EMBL) for their expert technical advice.